Initial activation of EpCAM cleavage viacell-to-cell contact

Author's response to reviews Title: Initial activation of EpCAM signalling via cell-to-cell contact Authors:

During contact activation of plasma, cleavage of surface-bound Hageman

During contact activation of plasma, cleavage of surface-bound Hageman factor (HF) 1 occurs at two closely situated sites, one within a disulfide loop, termed "site 1", and one outside the disulfide loop, termed "site 2" (1). The result of these cleavages is two forms of active HF (2) which we have given the names a-HF a and fi-HFa. Fig. 1 defines these two forms based on their derivation (last...

Effects of different activation protocols on cleavage rate and blastocyst production of caprine oocytes

The present study was undertaken to assess the effect of different chemical activators along with 6-DMAP on in vitro matured caprine oocytes. From 4332 ovaries, 14235 cumulus oocyte complexes (COCs) were collected which were matured in TCM-199 medium containing follicle stimulating hormone (FSH) (5 µg/ml), Leutinizing hormone (LH) (10 µg/ml), oestradiol-17β (1 µg/ml) supplemented with 10% fetal...

Initial contact guidance during cell spreading is contractility-independent.

A wide variety of cell types exhibit substrate topography-based behavior, also known as contact guidance. However, the precise cellular mechanisms underlying this process are still unknown. In this study, we investigated contact guidance by studying the reaction of human endothelial cells (ECs) to well-defined microgroove topographies, both during and after initial cell spreading. As the cytosk...

Cleavage of human high-molecular weight kininogen by purified kallikreins and upon contact activation of plasma.

To study the digestion pattern of human high-molecular weight (mol wt) kininogen (HMWK) in plasma during contact activation we have prepared monoclonal antibodies (MoAbs) to the light-chain (LC) and the heavy-chain moiety of HMWK. One MoAb from each set was purified, and neither MoAb inhibited the clotting activity of HMWK. In enzyme-linked immunosorbent assay and immunoblotting experiments nei...